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Induction of convulsions with kainic acid (KA) in rats can induce an increase in serum and ECF Zn but overall depletion of tissue Zn (271), while Zn appeared to accelerate brain infarction after focal ischemia in rats (272)

In vitro crotonylation assays For in vitro crotonylation assays, 5 g recombinant GST-tagged PGK1 were incubated with 100 M crotonyl-CoA, and 0.5, 1, or 2.5 g recombinant His-tagged PCAF at 30 C for 1 h in buffer A (50 mM Tris, pH 8.0, 10% glycerol, 1 mM DTT, 0.2 mM PMSF)