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Description

K562 cells were obtained from BCRC (Bioresource Collection and Research Center, Taiwan), and TCCS/KOPM28 from Takeshi Inukai (Department of Pediatrics, School of Medicine, University of Yamanashi, Chuo, Japan)

The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added

Therapeutic Strategy The im ediments to instituting a therapeutic strategy based on augmentation of a critical neurotrophic factor system for the treatment of PD are several