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The doubly charged GSSG precursor ion provided a significantly better detector response and was selected for MRM transitions, as previously reported by Sun et al

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The procedure to locate the voxel for the IFC was: (a) center the voxel at the first axial slice above the ventricles at the height of one third of the sagittal midline and at the width center of the left hemisphere, (b) from the sagittal view locate the center length of the voxel parallel to the inferior frontal sulcus and locate the length over the inferior frontal junction, and (c) from the coronal view, locate the top of the voxel parallel to the cortical surface