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Cells were washed with PBST three times and incubated with secondary antibody solution at room temperature for 1 h

At the cellular level, these macroscopic changes reflect underlying disruptions in hormone signaling, oxidative homeostasis, and carbon metabolism, with suppressed photosynthetic capacity (manifested through reduced leaf expansion, limited biomass production, and stunted growth) representing a key factor exacerbating heat stress impacts on plant performance and productivity (Secomandi et al., 2025)

Misra H, Fridovich I

Copper as a target for prostate cancer therapeutics: copper-ionophore pharmacology and altering systemic copper distribution