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At 414.605, ALS2 is defined as either transportation by ground ambulance vehicle, medically necessary supplies and services, and the administration of at least three medications by intravenous push/bolus or by continuous infusion, excluding crystalloid, hypotonic, isotonic, and hypertonic solutions (Dextrose, Normal Saline, Ringer's Lactate)

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After washing the chips, an 80 l solution of Cy3 equivalent dye-conjugated streptavidin was added to each well, and the array was covered with aluminum foil and placed in a dark room at room temperature for 1 h