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In flow cytometry, cells were collected and resuspended in 50ul FACS buffer (PBS + 2.5% FBS + 0.5% sodium azide) and TruStain FcX was used TM Human monocyte enrichment cocktail (Stemcell Technologies, Vancouver, Canada) monocytes were isolated from whole blood of healthy donors according to the manufacturer's instructions

For each well, the fold change in luminescence relative to the initial luminescence was measured and reported in a log 2 scale as the number of cell doublings

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