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For cell samples, cells were lysed with lysis buffer (50 mM Tris-HCl [pH 8.0], 1% Triton X-100, 0.5% Nonidet P-40, 10 mM dithiothreitol, 1% protease inhibitor cocktail, 150 mM NaCl, and 5 mM EDTA) on ice for 30 min, followed by centrifugation (12,000 g , 20 min, and 4 C)

*Based on a 20,000 IU dose with a total infusion volume of 35 mL at a rate of 2 mL per minute

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Even modest amounts of alcohol can turn a recreational GHB dose into a medical emergency, because both substances suppress the central nervous system at the same time