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In general, affinity chromatography can be effectively used to isolate a protein that recognizes group X by:covalently attaching X or a derivative of it to a column, adding a mixture of proteins to this column, which is then washed with buffer to remove unbound proteins, eluting the desired protein by adding a high concentration of a soluble form of X or altering the conditions to decrease binding affinity

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Replacing 50% of rice flour with coconut flour increased the moisture content (6.65%), fat (2.15%), protein (2.55%), and ash (0.38%) in the final product