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Nat Rev Mol Cell Biol (2007) 8(10):81324

NHS (diluted 1:200 in veronal buffer) (Complement Technologies), pure human C1q (diluted to 0.366 g/mL in veronal buffer) (Complement Technologies) or pure mouse C1q (diluted to 0.366 g/mL in veronal buffer) (Prospecbio) was added and the plates incubated at 37 C for 30 min