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Proteolytic activity assay To determine the activity of the proteases against Azocoll and Elastin Congo Red, 2.5 g of each KLIKK protease was diluted in assay buffer (50 mM Tris, pH 8.0, 2.5 mM CaCl 2 , and 0.02% NaN 3 ) to a final volume of 125 l, and then mixed with 125 l of substrate in assay buffer (15 mg ml 1 ), followed by incubation for 2 h (Azocoll) or 16 h (Elastin Congo Red) at 37C with shaking

Ignoring reconstitution accuracy Imprecise measurement during reconstitution creates concentration errors affecting every subsequent dose

This research ventured turned out to be disappointing because none of the tested strains of L

Protandim -supplemented mice showed a 33% reduction in skin tumor incidence and a 57% reduction in tumor multiplicity (Liu et al., 2009)