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To characterize the particle size distributions of inhaled e-cig aerosols, key factors determining their exposure through deposition, we employed a Scanning Mobility Particle Sizer (SMPS 3080, TSI, Inc., USA) and an Aerodynamic Particle Sizer (APS 3321, TSI, Inc., USA) to measure e-cig aerosols from the lung chamber at three discrete time points: after the first and last vaping puff, and following 5 min of ongoing ventilation (Fig

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Avoid strong suction: Inhaling forcefully can create pressure that may affect the filling, especially if pulp protection was used