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Marques, F

Ji X, Li C, Ou Y, Li N, Yuan K, Yang G, Chen X, Yang Z, Liu B, Cheung WW, Wang L, Huang R, Lan T (2016) Andrographolide ameliorates diabetic nephropathy by attenuating hyperglycemia-mediated renal oxidative stress and inflammation via Akt/NF-B pathway

For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)

however, minor transient alterations were notedhigher glucose and lower insulin levels in cord blood at birth, slightly higher adiposity at 18 months, and a trend toward a more atherogenic lipid profile (reduced HDL, elevated triglycerides, total cholesterol, and LDL) in the HPLGI group at later follow-ups [181]