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Some commenters broadly criticized our rationale for expanding the application of the transfer of care modifiers, stating that in modern medical practice it is common for a surgeon to direct a patient's overall post-operative care while the patient also sees other practitioners, for example, a primary care practitioner

Since patients who are HbS heterozygous and have around 30% circulating fetal hemoglobin seldom have the severe clinical course linked to SCD, this is expected to be effective [81,82,83,84]

After one hour, coverslips were washed in blocking buffer, and further labeled with Alexa Fluor 488-conjugated secondary antibodies (Invitrogen, Carlsbad, CA) for one hour at RT as above

This allows the copper to participate in various enzymatic reactions and cellular processes crucial for regeneration and repair