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Buffers used for the various steps in the solubility of IBs LSB-1: 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM Phenylmethanesulfonyl fluoride (PMSF) (Sigma, Catalogue-P7626) and 500 L BugBuster Protein Extraction Reagent (70,584 Merck-Millipore)

Western blot analysis RIN-m5f cells were lysed, and the protein contents of the resultant homogenates were quantified using a BCA protein assay kit (Beyotime Institute of Biotechnology)

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General symptoms include day-long fatigue, irritability, food cravings, insomnia, and exhaustion