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To further elucidate its mechanism in asthma-related ferroptosis, we performed virtual docking screening combined with molecular dynamics simulations, which revealed that luteolin binds effectively to ALOX15 and maintains the protein in a relatively stable conformation, thereby potentially mitigating ferroptosis in asthma by inhibiting ALOX15

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Unexpectedly, activation of p21, ICAM1, or SRXN1 was not observed (Supplementary Figure S5), although DNA (Nassini et al., 2010), and Keap1 ( Figure 6 ) is consistent with the lack of activation of an adaptive oxidative stress response pathway observed here and in a previous study (Tafazoli and OBrien, 2009)