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2.4.3 Flow cytometric analysis of stimulated PBMCs Stimulated and unstimulated PBMCs were labelled with a mixture of four conjugated surface antibodies including CD4/MaxLight405 (Cat#227418-ML405, USBiological Life Science, USA), CD8/Maxlight490 (Cat#214734-ML405, USBiological Life Science, USA), CD45RO/AlexaFlour647 (Cat# MCA2434A647, Bio-rad), and WC1/FITC (Cat# MA5-28518, Thermofisher) diluted in FACS buffer

As a constituent of simulated plasma protein mixture for Raman spectra analysis

It directly participates in methionine synthase reactions that convert homocysteine to methionine

This meticulously engineered research system is designed for investigators who demand high-fidelity data consistency in longitudinal studies observing cellular energy metabolism, receptor signaling, and the preservation of lean muscle tissue in various research models