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Glutathione - Synthesis SFRBM Education Program Dickinson et al.17 The Glutathione Cycle - 1 The processes of de novo GSH biosynthesis by GCL and GS

Hepatology 27 , 2834 (1998)

Sections were analyzed for the presence of free iron using a HEMATOGNOST Fe (SigmaAldrich, Germany, Catalogue number: 112084) staining kit for the detection of free ionic iron in cells according to the manufacturers instruction

De novo synthesis of serine from the glycolytic intermediate 3-phosphoglycerate (3-PG) occurs through a pathway consisting of three sequential steps: phosphoglycerate dehydrogenase (PHGDH) oxidizes 3-PG to 3-phosphohydroxypyruvate (pPYR) using an NAD + cofactor, phosphoserine aminotransferase (PSAT) transaminates pPYR to phosphoserine (pSER) using glutamate as the nitrogen donor, and finally phosphoserine phosphatase (PSPH) dephosphorylates pSER to produce serine [68]