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To confirm GSTP1 knockdown in A2780DPP cells, we isolated individual puromycin-resistant colonies 24 h after colony formation using cloning cylinders, and extracted sufficient RNA to confirm GSTP1 knockdown by qRTPCR analysis (Figure 3B)

Up to 350 mg per day from supplements appears safe, though exceeding this may cause diarrhea

Screening at the time of diagnosis and annually thereafter is important for detecting DN in a timely manner and preventing further damage to the nervous system

The HSYA + 2-ME group received HSYA (50 mg/kg, intraperitoneal) 1 h before CLP and 2-ME (10 mg/kg, intraperitoneal) 1 h after CLP