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Motif 3, 6, and 7 has been identified as PavGST conserved motifs and could be traced in all subfamilies which revealed that the addition of these three motifs (3, 6, and 7) in all subfamilies members occurred through some specific mutual evolutionary process and they have some specific function, while few subfamilies had exclusive motif formation, such as subfamilies I and II own unique motif 12 and 20, respectively, whereas motif 9 was traced only in VI and VII subfamilies which demonstrated that these two families faced some unique evolutionary processes, and these families have some unique functionalities