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Description

Anim Nutr 15:256274

Briefly, the liver tissues were perfused through the portal vein with Ca 2+ -free medium (Earles balanced salt solution) containing EGTA (0.5 mM) and then with the same medium without EGTA, finally with the perfusate containing collagenase (Type IV, 0.25 mg/ml) and Ca 2+ at physiological concentration (2 mM)