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The labeled protein concentrations were adjusted to 100 nM based on fluorescence intensity measured during pretest scans, ensuring signal quality and uniformity across all three Syn constructs for direct comparison of binding affinities

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The peptide drug class encompasses everything from blockbuster GLP-1 receptor agonists prescribed to millions of patients, to emerging compounds still building their evidence base in clinical research

jejuni (Cj) to a CFE reaction to glycosylate a cell-free synthesized protein containing a glycosylation tag