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Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 100 g/mL penicillin/streptomycin/gentamicin (Invitrogen, Gibco) and in the condition of 95% air and 5% CO 2 humidified atmosphere at 37 C

Rera M, Bahadorani S, Cho J, et al

Moreover, viral PAMPs and ROS activate the TLR4NF-B pathway, driving the release of pro-inflammatory mediators such as TNF- and IL-1, thereby exacerbating epithelial barrier dysfunction