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Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 340 nm at 25C Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions 15 mL conical tubes Incubator at 25C Deionized water Assay Procedure Summary Prepare Working Solution

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Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and thiobarbituric acid reactive substances (TBARS) were measured using ELISA kits according to the company guidelines