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Luciferase activity from transfection (-GnHCl), which measures RNA replication, was comparable in the cell lysates co-infected with CAV20-wt or CAV20-BSOr viruses (Figure 5C), confirming our preliminary finding that GSH-depletion has no inhibitory effect on RNA replication (Figure 5B)

I used a 12-cup one, but mine was a bit old and battered and wasn't not as non-stick as I needed it to be

Discussion Among the questions we set out to address was why RHEBGTP, which is critical for GF-dependent activation of mTORC1 in cells 3,5 , is such a low-affinity activator in solution 4