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All personnel involved in the testing procedure were kept unaware of the casecontrol status of the samples, maintaining strict blinding throughout the analysis

Cells were placed in an IncuCyte S3 live-cell analysis system (Essen BioScience, Ann Arbor MI, USA) or a CellCyte X Live Cell Analyzer (Cytena, Freiburg im Breisgau, Germany) housed in a humidified incubator containing 95% air and 5% CO 2 at 37 C

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Practical strategies for maintaining electrolytes on semaglutide include choosing the best foods to eat on semaglutide with an emphasis on mineral-dense options, and being deliberate about electrolyte supplementation on days when food intake is low