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Western blotting analysis Hepatocytes were lysed in RIPA buffer, and the isolated proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45 m polyvinylidene difluoride membranes

and 72.9%, 52.1%, 50.8%, 39.7%, and 36.9% for NoDS, respectively ( p 80% of both categories currently follow 1-19 patients with LT- ineligible BCLC-D HCC