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Following incubation, each sample was filtered rapidly under vacuum through glass fiber filters presoaked in a buffer and rinsed several times with an ice-cold buffer using a cell harvester

However, by failing to bolster the barrier function of tracheal, bronchi, bronchiole and more distal airway surfaces, these nasal technologies do not provide comprehensive coverage of the airway epithelium

Notably, weight loss was accompanied by an increase in the abdominal circumference, which may indicate a systemic inflammatory response secondary to chemotherapy, possibly linked to hypercortisolism

However, there is no evidence that enforcing reduction of these sensors is sufficient for their activation, meaning there would then be no reason to expect stimuli, such as DCA or GPX impairment, that elevate GSH to activate the UPR on their own