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The endogenous release of RNS, such as nitric oxide (NO ), is produced from L-arginine in reactions of catalyze by three main isoforms of nitric oxide synthase (NOS): epithelial NOS, neuronal NOS and inducible NOS, which are activated in response to various endotoxin or cytokine signals ( and form highly reactive molecules such as ONOO (Salisbury and Bronas, 2015

The following antibodies were used for detection: C/EBP (E299) from Abcam, -Tubulin (GT114) from GeneTex and -actin (clone C4) (#691001) from MP Biomedicals

Although the measurement of plasma antioxidant capacity lacks specificity, a reduction in plasma antioxidant capacity suggests oxidative stress, while an increase may indicate an early response to inflammation (Brock et al., 2004)

10.1007/s11120-005-8425-1 30 MicheletL.ZaffagniniM.MarchandC.CollinV.DecottigniesP.TsanP.et al