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Combination antioxidant therapies are frequently favored by clinicians based on the hypothesis that synergistic effects may better restore redox balance and improve sperm function [39]

For double transductions (GLUT3 allele + shGLUT3), unmodified THP-1 cells were serially transduced first with the GLUT3 expression plasmid by puromycin selection followed by the shGLUT3 allele by hygromycin selection