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In whole-SPZ ELISA, 10 4 SPZs/well or salivary glands insoluble fraction (equivalent to those that yielded 10 4 SPZs) from uninfected mosquitoes were fixed to the wells of microtiter plates and overlayed with either 50% HIS (heat inactivated serum) in VBS ++ (1x veronal buffered saline, pH 7.3, 0.15mM CaCl 2 , 0.5mM MgCl 2 ) or VBS ++ alone

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The design of trials for IBS treatments, as outlined by the Rome criteria, highlights that placebo effects pose a significant challenge, particularly when the endpoints evaluated are subjective

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