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C20:2, a long-chain PUFA (LC-PUFA), was significantly elevated in the 1:1 Lys/Met group, suggesting that this ratio enhances the efficiency of linoleic acid (LA) and -linolenic acid (ALA) conversion, modulating key lipid metabolic pathways (40)

2.3 Phenotypic trait data collection In terms of growth performance, initial body weight (IBW) and final body weight (FBW) were recorded at the beginning and end of the experiment, respectively, while average daily gain (ADG) and net gain (NG) were calculated subsequently

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