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Mitochondrial trafficking in neurons

Finally, the purified structure was annealed with 10X excess of the oligonucleotide 253.Primer_for_Dig that was partially complementary to the oligonucleotide 386.Template hairpin 45dC, by heating at 95 C for 1 min and cooling down from 80 C to 10 C at a 0.5 C 10 s -1 rate in annealing buffer (10 mM Tris-HCl pH 7.5, and 1 mM MgCl 2 )