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At the same time, the drastic reduction of serum testosterone levels induced by ADT produces multiple side effects such as bone fracture, cardiovascular disease, sexual dysfunction anemia

Certain of these previous reports were able to pinpoint the specific cysteine residues which were modified in the GSTs, namely Cys50 in rat and human MGST1 (Shin et al., The analysis of proteins and their modifications benefits from continual advancements in liquid chromatography-tandem mass spectrometry (LC-MS/MS), a broadly used tool for proteomic investigations (Aebersold and Mann, In this work, several analytical approaches (Figure 2) were investigated combining different techniques for sample preparation and LC-MS/MS analysis to identify APAP-related covalent binding to purified human GSTs (overview in Table 1, note: Met1 is considered here as the first amino acid in protein sequences throughout the whole manuscript)

In this model, 95.4% of the residues were in favoured regions of Ramachandran plot, and all the others were in allowed regions

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