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Once outside the cell, GSH degradation occurs exclusively in the extracellular space and only on the surface of cells that express the ectoenzyme -glutamyl transferase

Analytical Techniques Gas ChromatographyMass Spectrometry The first step was the derivatization of glutathione and analysis of N , S -ethoxycarbonyl methyl esters (NSECM) by the GCMS method

Such therapeutics would be mimetics of the pathogen-binding domains of host complement regulators, preventing pathogens from hijacking host complement or eliciting complement regulators to inhibit complement activation (50)

The harvested cells were washed once in PBS, pH 7.4, and then resuspended in PBS containing 400 g of proteinase K (Sigma-Aldrich) and incubated at 22 C for 1 h with gentle agitation every 5 min