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Ramos-Tovar E, Muriel P (2023) NLRP3 inflammasome in hepatic diseases: a pharmacological target

After overnight incubation, cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilised with 0.2% TritonX-100, blocked with 5% of goat serum and incubated overnight at 4 C with the anti-mature IGF-1 antibody (Prepotech)