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Further, the temperature was increased to 110C and it was incubated for 24 h for complete hydrolysis of the protein sample ( Yellow pigment estimation The estimation of yellow pigment (-carotene) in the sample was carried out as per the modified method of -1

Voura EB, Ramjeesingh RA, Montgomery AM, Siu CH

On occasion, similar fibroobliterative cholangitis can be seen in 1) PBC, 2) mechanical obstruction of larger bile ducts, 3) ductopenic rejection following liver transplantation, and 4) after intraarterial infusion of 5-fluorouracil

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