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Now the injection needle ( I use a 25G x 1inch ) is put on the syringe and any air left in the syringe is expelled until a tiny bead of B12 appears on the tip of the needle

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Therefore, in order to investigate metabolism in cultured neurons and astrocytes, these cells can be incubated in the presence of 13 C-labelled substrates and the percent 13 C enrichment determined in various metabolites, thereby mapping the pathways