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Phosphorylation is the common modification of the proteins, which often alters various properties of proteins, including subcellular localization

MEFs were grown in Dulbeccos minimum essential media (DMEM, Invitrogen) supplemented with 10% FBS (Sigma) and antibiotics (Sigma) in a humidified incubator at 37 C with 5% CO 2

ThT (Thioflavin-T Fluorescence): Binding of the planar dye Thioflavin-T to amyloid-like aggregates increases its fluorescence emission intensity at ~485 nm when excited at 445 nm

Risk of Injury (Slips and Falls) Risk : Cold showers may cause muscle stiffness or shivering, potentially impacting balance or coordination