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Additionally, carbon units for the folate cycle can also be derived from formate, which can donate one carbon directly to tetrahydrofolate in a reversible reaction catalyzed by the tri-enzyme Methylenetetrahydrofolate Dehydrogenase, Cyclohydrolase, and Formyltetrahydrofolate Synthetase 1 (MTHFD1)

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Methylfolate helps power the methylation cycle, which regulates: Homocysteine metabolism Neurotransmitter production (serotonin, dopamine, norepinephrine) Hormone regulation Detoxification and antioxidant production DNA synthesis and repair When the MTHFR gene has a variant (like C677T or A1298C), the enzyme works less efficiently