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Determination of mitochondrial reactive oxygen species production The production of reactive oxygen species (ROS) in the mitochondrial matrix was determined as a time-dependent increase in the fluorescence intensity of selective MitoSOX (Life Technologies, Pleasanton, CA, USA) dye in the cells exposed to tested therapeutics using flow cytometry (BD LSRII, BD Biosciences, San Jose, CA, USA)

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This is likely because monoclonal antibodies have a poor brain penetration which prevents them from acting on the compartmentalized inflammation in PPMS

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