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CellTiter-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)

The injection delivers the vitamin directly into your bloodstream for faster and more efficient results

This method, however, cannot account for misremembering the exact LMP, nor irregularities in ovulation and menstruation, so additional methods, like ultrasounds, are also used to calculate gestational age