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SFN induces a pro-oxidative milieu in untransformed human T-cells ROS are ubiquitously generated and recognized as important signaling molecules for T-cell activation, but excessive ROS generation or prolonged exposure to high ROS concentrations impair T-cell functions ( 2 DCFDA and treated with SFN prior to flow cytometric analysis

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After centrifugation at 18,000 rpm for 30 min, the supernatant was collected and incubated with glutathione-Sepharose 4B at 4 C, followed by washing with PBS buffer