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Western blotting (WB) and EGFP fluorescence were used to detect transgene expression levels and distribution after 4 weeks (Fig S2CE)

10.1371/journal.pone.0269619 54 HimbertS.QadriS

These modifications reduce its affinity for insulin-like growth factor binding proteins, particularly IGFBP-3, which normally sequesters a large majority of circulating native IGF-1