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Scheller, J

Premature senescence of vascular cells is induced by HIV protease inhibitors: implication of prelamin A and reversion by statin

For immunoprecipitation, cells were harvested and lysed in NP-40 lysis buffer (50 mM HEPES, 150 mM NaCl, 10% glycerol, 1% NP-40, 1 mM EDTA, 1 mM EGTA, pH 7.4) supplemented with protease and phosphatase inhibitor cocktail