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After final alkylation, all samples were diluted (1:1) with 20% ACN/0.1% FA containing 50 nM of deuterated internal standards and analyzed by LC-MS

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( i ) Confocal images of mitochondria (imaged by TOM20 staining) and mtDNA in cells treated with 5FU for 72 h

Unlike regular glutathione, liposomal glutathione supplements do not break down easily in the digestive system and can ensure better absorption and bioavailability