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After three washing, diluted streptavidin- Horseradish peroxidase (HRP) was added to each well and plate was incubated for 60 min at room temperature

Briefly, the mitochondrial pellet was resuspended in lysis buffer consisting of 10 mmol/L KH 2 PO 4 (pH 7.4), 1 mmol/L PMSF, and protease inhibitor cocktail, and the mixture was shaken at 4 C for 15 min

Compared to GLP-1 Agonists: Weight management peptides like semaglutide and tirzepatide have demonstrated weight loss efficacy superior to AOD-9604 in clinical trials, but they also have distinct side effect profiles