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Internal reference standards sulforaphane-d8 (50 M) and sulforaphane-sulforaphane-d8-N-Acetyl-L-cysteine (10 M) were added to each well of the collection plate before eluting the samples with 0.2% AA in methanol

Circulation 130:1826 Ali-Hassan-Sayegh S, Mirhosseini SJ, Rezaeisadrabadi M et al (2014) Antioxidant supplementations for prevention of atrial fibrillation after cardiac surgery: an updated comprehensive systematic review and meta-analysis of 23 randomized controlled trials

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However, it is worth noting that the acetyl-CoA concentrations used (1-10 mM) in vitro represent approximately 10-100 fold molar excess over total cysteine residues in GAPDH, conditions that favor non-enzymatic acetylation