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The same principle of using an immobilized substrate for enzyme purification was adopted for the enrichment of lipase by using powdered stearic acid, the purification of pepsin by employing edestin (a crystalline protein), and the isolation of porcine elastase by using powdered elastin [2427]

This growth is often triggered by a lack of oxygen (ischemia) or inflammation

MS mode was set to positive detection and a mass range of 8003000 m/z

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