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By combining multi-omics technologies such as metabolomics, single-cell transcriptomics and spatial omics, we systematically depict the dynamic changes of GSH in different tissues (such as liver, brain, tumor tissue, etc.) and under different disease states (such as normal, inflammatory, cancerous, etc.), and deeply analyze the functional mechanism of GSH in different physiological and pathological backgrounds, providing a more comprehensive theoretical basis for the diagnosis, treatment and prognosis of diseases