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The 176-191 fragment without the tyrosine addition is the unmodified native sequence

While singly-housed animals may experience stress that impacts metabolism, body weight, and adiposity 65,66,67,68,69 , the singly-housed animals in this study were treated identically (to the best of the experimenters ability) to minimize and equalize stress across all animals and reduce confounding variables

Losing weight can be difficult when you dont seem to have the energy to keep up with your exercise routine and that goes double when it doesnt seem like youre making any progress because of a slow metabolism

Maintain the supporting factors that affect outcomes